Collabera Research Scientist in Boston, Massachusetts

Research Scientist

Location:Boston, MassachusettsUS

Posted On:3/13/2018

Job Code:2492RSMA

Job Description

The Biologics Discovery Department is seeking a biochemist with deep expertise in E.coli protein expression and purification including purification from inclusion bodies.

Category:Non-IT code:new

Area Code


Job Requirements


• B.S. Required, M.S. preferred 3-5 years.

• Experience in protein purification from E.coli inclusion bodies using refolding, including generation of inclusion bodies

• Experience in affinity purification, ion exchange chromatography and preparative size exclusion using Unicorn software

• Basic protein analytics including analytical SEC, SDS-PAGE and LAL assay

• Ability to run multiple purifications in parallel and scale up purification to multiple liters of cell growth

• Basic molecular biology skills including plasmid transformation and growth and induction of E.coli


• Expression and purification from mammalian cells is preferred but not required

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