Collabera Research Scientist / Scientist in Boston, Massachusetts

Research Scientist / Scientist

Location:Boston, MassachusettsUS

Posted On:3/12/2018

Job Code:Sci2784MA

Job Description

The Biologics Discovery Department is seeking a biochemist with deep expertise in E.coli protein expression and purification including purification from inclusion bodies.

Category:Non-IT code:new

Area Code


Job Requirements


• B.S. Required, M.S. preferred 3-5 years. • Experience in protein purification from E.coli inclusion bodies using refolding, including generation of inclusion bodies • Experience in affinity purification, ion exchange chromatography and preparative size exclusion using Unicorn software • Basic protein analytics including analytical SEC, SDS-PAGE and LAL assay • Ability to run multiple purifications in parallel and scale up purification to multiple liters of cell growth • Basic molecular biology skills including plasmid transformation and growth and induction of E.coli / Preferred: • Expression and purification from mammalian cells is preferred but not required

Preferred Skills: