Collabera Research Scientist / Scientist in Boston, Massachusetts
Research Scientist / Scientist
The Biologics Discovery Department is seeking a biochemist with deep expertise in E.coli protein expression and purification including purification from inclusion bodies.
• B.S. Required, M.S. preferred 3-5 years. • Experience in protein purification from E.coli inclusion bodies using refolding, including generation of inclusion bodies • Experience in affinity purification, ion exchange chromatography and preparative size exclusion using Unicorn software • Basic protein analytics including analytical SEC, SDS-PAGE and LAL assay • Ability to run multiple purifications in parallel and scale up purification to multiple liters of cell growth • Basic molecular biology skills including plasmid transformation and growth and induction of E.coli / Preferred: • Expression and purification from mammalian cells is preferred but not required